Composite
CHOP

Part:BBa_K2290000:Design

Designed by: Joseph Harrison   Group: iGEM17_East_Chapel_Hill   (2017-10-19)


Fluoride Riboswitch regulated Chloramphenicol Acetyltranferase Operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 31
    Illegal NheI site found at 54
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 182
    Illegal XhoI site found at 887
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To use this part with the fluoride riboswitch one needs to use the delta-crcB E. coli strain, available from the Yale E. coli Genetic Stock Center. This allows fluoride to accumulate inside of E. coli otherwise the E. coli will export fluoride.


Source

We ordered this operon from IDT DNA

References